Optimizing Analytical Methods to Determine Lycopene Levels in Syrian Tomatoes from Various Regions

Background: The particular attention in recent years on high nutritional quality food is increasing because of its health benefits. Tomato, which a common fruit different regions Syria, one these foods. It contains plentiful antioxidant compounds, including lycopene. Lycopene plays very significant role human and nutrition; it considered strong due to ability trap singlet oxygen remove the peroxyl radical. This study aims quantify lycopene content fresh tomatoes Syria determine best analytical method (solvent for separation by HPLC) HPLC. Methods: Tomatoes from Syrian were quantitatively analyzed detection lycopene, using reversed-phase performance liquid chromatography through isocratic elution UV detection. Many mobile phases under experiments order maximum achievement separation. Result: An Analytical determination tomato samples was optimized proper solvent (MeOH/propanol/THF) (15:65:20). ranged 55.94 103.70 ìg/g, this disparity attributed many genetic environmental factors. suggests that rich source major provenance powerful can be as possible alternative antioxidative supplements.